Terminator

Part:BBa_K3768004:Design

Designed by: Gabriel Aguiar-Tawil   Group: iGEM21_Concordia-Montreal   (2021-10-19)


CPS1 Terminator for S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the high amount of As and Ts present in the sequence, it may be difficult to amplify this gene using PCR. After using a high fidelity polymerase, we were not able to amplify this part, however, we were able to obtain it after changing to a lower fidelity polymerase.


Source

This part comes from the S. cerevisiae genome, meaning that it can be amplified from a genomic prep.

References

Curran, K. A., Karim, A. S., Gupta, A., & Alper, H. S. (2013). Use of expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications. Metabolic Engineering, 19, 88–97. https://doi.org/10.1016/j.ymben.2013.07.001